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Old 09-23-2016, 05:28 AM   #1
CHObot
Junior Member
 
Location: Greater Boston Area

Join Date: May 2013
Posts: 8
Default minIon for plasmid sequencing

I was just wondering if anyone has used the minIon for plasmid sequencing? I suppose it might be overkill and a waste or reagents for such a small task. But I am asking because I have large plasmids to sequence (in the 18 to 20 kb range), and the plasmids contain several copies (up to as many as 4 copies) of certain sequences. So using sanger sequencing is costly and labor intensive and we end up with reads that could align to more than one spot on the plasmid making it hard to tell if and where a mutation has occurred. I am also exploring Illumina based methods, but am curious about the nanopore technologies. Seems like a 20 kb plasmid could simply be linearized and the entire molecule sequenced in a single read.
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