i have short solexa sequences mapping to genome, in some positions there're
some certain enriched mutations, how do i know whether this mutation is non-synonimous or not ?
one method is download transcript isoforms and coding annotations from ucsc, then count from the coding start site to determine what codon this position belong to then go to codon - Aa table t ocheck, can anyone give me better method ? thanks in advance
also, i have checked, in biomart we can download phase information ~
maybe i will use this
ZSL
some certain enriched mutations, how do i know whether this mutation is non-synonimous or not ?
one method is download transcript isoforms and coding annotations from ucsc, then count from the coding start site to determine what codon this position belong to then go to codon - Aa table t ocheck, can anyone give me better method ? thanks in advance
also, i have checked, in biomart we can download phase information ~
maybe i will use this
ZSL
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