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Old 02-03-2018, 07:57 PM   #1
icahn_seq
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Location: NYC

Join Date: Feb 2018
Posts: 2
Default SureSelect XT Probes

Hi everyone,

I was wondering if anyone else had tested their SureSelect XT (< 3.0 Mb bait territory) bait library concentration?

I recently used the Qubit to test mine and it was ~ 50 ng/uL. I'm a little confused because according to the original Gnirke et al Nat. Biotech 2009 papers hybridization was done with equal mass of pond library and bait library (500 ng each). Going by the XT protocol wouldn't this mean it would be only 100 ng bait to 750 ng library?
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