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Old 04-16-2009, 05:24 AM   #4
jOn
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Location: UK

Join Date: Oct 2008
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For the reasons already pointed out by kmcarr

"Compared to genomic DNA sequencing cDNA sequencing generally produces fewer filter passed reads (more failures due to mixed reads) and the mean length is shorter. There is also more variability from library to library which probably is a DNA quality issue."

I would advise doing a comprehensive titration for each cDNA sample processed. Also if you're going ahead with normalization you'll notice a deffinate shift in fragment sizes when run on a gel.
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