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Old 05-15-2013, 03:30 PM   #36
carmeyeii
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Location: Mexico

Join Date: Mar 2011
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Quote:
Originally Posted by pmiguel View Post
But it obscures a host of library issues that otherwise would have to be dealt with directly. Not the least of which is that some characteristic of the TruSeq adapters in TruSeq Prep kits makes them perform extremely poorly for clustering without at least 1 cycle of "enrichment" PCR.
Thanks, Phillip,

I wonder, though, if that has changed since the new Y adapters already contain the flowcell-complementary sequence?

Carmen
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