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Old 07-14-2016, 09:50 AM   #1
Junior Member
Location: UK

Join Date: Mar 2013
Posts: 7
Default Custom multiplex sequencing barcodes / indexes (8-mer)

Hi all,

I have synthesised a custom set of Illumina adaptors that use an 8-mer index to allow 384-plex multiplexing. I got these index sequences from the Nextera XT 384-plex index kit.

I am looking to expand my index set beyond 384-plex. Preferably beyond 1000-plex. Does anyone have the sequences for an index set, with good balance, that would allow me to expand my custom adaptor multiplex size up to 1000 (or beyond)? I would prefer if the indexes were 8-mers but could try longer indexes if this allow expansion of the set...

Thank you in advance for your help.

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