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Old 06-13-2016, 03:09 AM   #4
Markiyan
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Location: Cambridge

Join Date: Sep 2010
Posts: 101
Lightbulb Nextera matepair tends to have inserts from 2kb to 12kb.

Nextera matepair tends to have inserts in the 2kb to 12kb range.

So one library can be one size fits all...

If you add a bit more DNA (2x), it will become 3.5kb to 17kb (provided your ligation still works well).

In any case do one PCR-free library and one matepair one, and sequence them on 2x250 or 2x300 bp mode. (1-2 MiSeq runs).

It may be very tempting to go with hiseq 2x125bp, but 100X 2x125bp gives way worse assembly than a good 20x 2x250 or 2x300 bp with PCR-free library...
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