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Old 05-19-2017, 10:45 AM   #3
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

Has anyone bought a "Nanodrop One"? It is supposed to be able to analyze your spectrum of your sample and deconvolute the spectra of nucleic acids, protein, guanidine and phenol. That is, supposedly it can correct for the presence of the latter three common spectral contaminants of nucleic acid preps/methods and give you an accurate nucleic acid concentration.

I don't imagine it will be able to distinguish between RNA and DNA, so genomic DNA prep concentrations will still usually be wrong. But for RNA preps, DNA usually isn't a major contaminant.

If it works and renuka22 had one, it would have saved him or her some troubles...


Last edited by pmiguel; 05-19-2017 at 11:12 AM.
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