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Old 05-20-2017, 12:17 AM   #6
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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RIN number is valid only for total RNA and one of the main factors in its calculation is 18S/28S ratio. With a successful polyA selection rRNA would be excluded so RIN number is not meaningful. If after polyA selection you still see 18S or 28S bands then it has been less successful. See Agilent application note about RIN number: https://www.agilent.com/cs/library/a...989-1165EN.pdf
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