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Old 12-07-2008, 10:16 PM   #3
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Location: UK

Join Date: Jul 2008
Posts: 4

Thanks cjohns. We dont see any 80bp or 120b products in these libraries (our DNA is 150-300bp) but maybe there is a causative connection there somewhere. I guess if there were sequences which are complementary to the oligos on the FC but which didnt for some reason contain both amplification primer sequences (or the sequencing primer target) they could mop up the FC oligos and yield nothing. Maybe we need to clone and sequence this stuff...
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