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Old 08-25-2009, 05:52 AM   #4
Location: Germany

Join Date: Aug 2009
Posts: 33

This sounds a bit like it could be ssDNA filling up your FC, like you suggested. I've heard of similar things before, although I'm not sure where that amount of ssDNA comes from (preferred amplification of one strand??). That would at least explain the concentration difference you see with qPCR vs. PicoGreen.
However to get rid of ssDNA ?? - I thought the gel extraction should do the job, as ssDNA migrates slower than dsDNA.
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