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Old 09-01-2015, 05:35 PM   #15
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,189
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Quote:
Originally Posted by chariko View Post

I checked Spin-X from Costar (http://www.sigmaaldrich.com/labware/...ePage=17192542) and they only have 0.22 or 0.45 um filter tubes. Illumina┤s recommendation has 5 um.
In this step I am just filtering out the gel pieces but I wonder they recommend 5 um in order not to clog the porus thing that could happen in case you use a 0.45 um filter tube. Do you think this could happen? How could you avoid this clogging?
NEB kit recommends using 0.45 Ám gel filtration columns (same as the one you are pointing) with the same gels as recommended by both Illumina and NEB. They instruct to centrifuge the filter for 2 min @13.2k rpm.
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