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Old 03-02-2017, 04:45 PM   #5
luc
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Location: US

Join Date: Dec 2010
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Something in this description seems wrong. After bisulfite conversion the DNA should be (mostly) single stranded (since the bisulfite conversion requires single stranded DNA). Thus the standard end-repair, A-tailing and Illumina adapter ligation with Y-adapters will not work.

Quote:
Originally Posted by zubr View Post
This is what I could extract from core lab personnel:

1- Kit or method used for library prep

Genomic DNA was extracted from tissue, BS treated, sonicated, end repaired, dA-tailed. Then standard illumina adaptors were used for PE sequencing.
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