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Old 11-12-2012, 06:49 AM   #26
TonyBrooks
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Location: London

Join Date: Jun 2009
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Quote:
Originally Posted by earonesty View Post
PacBio reads, at Q8, are still quite useful for scaffolding assemblies. Crazy to hold back data when there are lots of people who could make use of long Q14 reads right now.
The problem is that scaffold runs for de novo are so niche in the grand scheme of things. The real money will be in resequencing, RNA-Seq, ChIP-Seq etc. As a core group, I'd say 99% of things we get asked to do fall into those categories. There's still a long way to go for ONT until it becomes a viable alternative.
Q14 is only really of value when you have a bunch of short read Q30s you can pin to the scaffold.
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