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Old 06-13-2018, 01:54 AM   #4
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,199

It should be enough for annealing low diversity short fragments such as adapter oligos. Denaturing should not destroy the DNA as in PCR this happens in every cycle. The fact that your fragments are not detected on Tape indicates that they have not been re-annealed. This is the reason that I suggested to do 1-2 PCR cycles.

Doing 2 cycles on majority of your library (denatured) will be better than several cycles of diluted library.
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