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Old 11-04-2016, 02:00 AM   #2
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Location: Germany

Join Date: Aug 2012
Posts: 2
Default Uneven number of biological reps on DEseq.

You should have at least three replicates for each sample. I had something similar happen to me with the Genomics Center sequencing one of my samples twice and one sample not at all...In any case, I ultimately re-submitted the missing sample. The PCA plots and differential expression results did not change in any meaningful way. Best of luck.
MatthewHaas is offline   Reply With Quote