Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #16
    If you do happen to perform such a comparison, please do post a link to it here and/or on biostars, since I expect many people would find it interesting.

    Comment


    • #17
      Originally posted by Zimbobo View Post
      Hello,

      does anyone know of any software that produces simulated RNA-Seq data. I am interested in questions like how many reads are needed for a good assembly with velvet for example, what read errors produce which problems in the assembly. Thanks in advance for any pointers.
      Why not download a real data set from NCBI and then randomly sample from that to derive pseudo-data sets of varying read depths? That way you would have an realistic baseline to compare to.
      Michael Black, Ph.D.
      ScitoVation LLC. RTP, N.C.

      Comment


      • #18
        Originally posted by mbblack View Post
        Why not download a real data set from NCBI and then randomly sample from that to derive pseudo-data sets of varying read depths? That way you would have an realistic baseline to compare to.
        Note that the person you're replying to posted that ~4 years ago...

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM
        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        30 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        32 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        28 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        53 views
        0 likes
        Last Post seqadmin  
        Working...
        X