I'm using the Tru-seq Stranded mRNA Sample Preparation kit and I made the mistake of not removing the RNA from the RNA Purification Beads before starting the First strand synthesis and then second strand synthesis. The only step I missed was removing the beads, I added everything else and ran the incubations required for the first and second strand synthesis. Is everything okay to continue or will I have to start over?
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
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03-08-2024, 10:41 AM -
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