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Old 03-30-2010, 12:21 PM   #3
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Location: New orleans

Join Date: Feb 2010
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Originally Posted by peromhc View Post
What stage of the library prep are you at?? Is this the final purified library? What size bands are you selection?
It is purified library for subsequent cluster generation in mRNA-seq. This library is generated by PCR enrichment during which the gel-seclection size at 200 bp is used as template. The PCR cycle is 15. Thus, in theory and according to illumina protocol of sample preparation for single end RNA-seq, the band shoud be only at around 200 bp, and the 500 bp band should not occur. I do not know why 500 bp band is yielded? I need 200 bp band.
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