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Old 01-08-2014, 10:50 AM   #4
Senior Member
Location: East Coast USA

Join Date: Feb 2008
Posts: 6,978

If you are sure that the index read has worked and for some reason the demultiplexing did not then you can:

a) Try to re-run the analysis with MiSeq reporter or if that does not work
b) try doing the basecalling/demultiplexing using bcltofastq on a separate unix server (for ref:

In any case first see what Tech Support says (I see that you are in Chile but hopefully there is a way to contact the tech support either local or the one in US).
GenoMax is offline   Reply With Quote