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Old 01-08-2014, 11:28 AM   #8
GenoMax
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Location: East Coast USA

Join Date: Feb 2008
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You can push cluster numbers beyond the recommended limits (http://www.illumina.com/systems/mise...fications.ilmn) for normal sequencing but in case of multiplexing one runs the risk on losing the run (like in this case, even though actual sequence reads are probably acceptable).

If these are amplicons you should stay within (lower end of) the recommended limits (800-900 clusters/mm^2 for V2).

Last edited by GenoMax; 01-08-2014 at 11:36 AM.
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