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Old 01-27-2014, 12:06 AM   #2
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Location: Cambridge, UK

Join Date: Sep 2009
Posts: 620

Since your sequence ends in ... GATC-s-T, you need to use its reverse complement, thus starting with AGATC.. This means you should be able to run Trim Galore in its default mode without specifying any -a because it is using just that sequence anyway.
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