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Old 01-27-2014, 12:21 AM   #3
foolishbrat
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Location: South East Asia

Join Date: Nov 2008
Posts: 44
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Hi, Thanks.

But the reverse complement of

5 ́-CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3 ́

Is this:
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG

I.e. it doesn't start with "AGATC".
Or trim_galore default adapter AGATCGGAAGAGC is no substring of the reverse complement above.

Did I miss anything?

Truly need your advice.
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