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Old 01-27-2014, 12:24 AM   #4
fkrueger
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Location: Cambridge, UK

Join Date: Sep 2009
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You need to add an A to the start of the reverse complemented sequence which is a result of the A-tailing process in the Illumina library preparation protocol. Then the start of both sequence will match up perfectly which is what your want to use. Hth
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