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Old 01-27-2014, 12:45 AM   #7
Location: South East Asia

Join Date: Nov 2008
Posts: 44

Originally Posted by fkrueger View Post
Since your sequence ends in ... GATC-s-T, you need to use its reverse complement, thus starting with AGATC.. This means you should be able to run Trim Galore in its default mode without specifying any -a because it is using just that sequence anyway.

You save my life. If you don't mind one last question.
How can I know whether or not to use the reverse complement adapter for trimming?

Any other alternative than GATC-s-T ending?
foolishbrat is offline   Reply With Quote