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Old 01-25-2016, 02:41 PM   #4
kerplunk412
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Location: Bioo Scientific, Austin, TX, USA

Join Date: Jun 2012
Posts: 119
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I say re-run it and see what happens. With something this strange I always like to make sure the result is repeatable before looking too much into it.

Also, if you still have the rest of the 1:20 dilution run it on an agarose gel and see if the banding pattern repeats. There is a chance something strange happened during the dilution.

Edit: One more thing. Was anything of this size (or expected size) run on the same chip? Maybe the well got double-loaded.
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