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Old 02-10-2017, 06:22 AM   #8
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
Posts: 503
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1) What is the organism that you're studying? And are there any related genomes that might be useful for comparison?

2) Is there any possibility that the sample might be heterogeneous/contaminated, and that the 750kbp segment is from a different species or symbiont? It might be informative to compare the GC content of that segment to the remainder of the chromosome. Kmer plots and BLAST searches might also prove useful.

3) And any chance that the Illumina dataset was normalized?
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