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Old 08-02-2019, 02:30 PM   #8
Bukowski
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Location: Aberdeen, Scotland

Join Date: Jan 2010
Posts: 388
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Quote:
Originally Posted by Haiqu View Post
My intro in the Introductions section clearly states that I'm retired and consider this a hobby activity. Sorry if I seemed too knowledgeable for that to come through here, it's a side-effect of being smart.
No one digs through your post history to answer your questions.

The Ion Torrent workflow is notoriously finicky. Many people submit DNA or pre-made libraries to service providers in other countries in order to get their sequencing done. And outside of professional contexts too. The economic feasibility of this has already been outlined, let alone the issue of tying yourself to a dead platform (I'm interested in how you would source reagents to run a PGM).

Are you sure a MinION and some Flongles wouldn't be a more cost effective route? I bet there's a lot of DIYbio people floating around that would ameliorate the cost of a starter pack. And the library prep workflow is a lot less complex.

Last edited by Bukowski; 08-02-2019 at 02:37 PM.
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