Has anybody come across studies that have looked into the optimal insert sizes for RNA-seq libraries?
Would you have recommendations? I assume the optimal size ranges might change with library prep protocols. I am especially interested in recommendations for protocols using RNA fragmentation, random-hexamer-primed 1st strand synthesis, and dUTP incorporation for strand specificity.
Thanks a lot in advance!
Would you have recommendations? I assume the optimal size ranges might change with library prep protocols. I am especially interested in recommendations for protocols using RNA fragmentation, random-hexamer-primed 1st strand synthesis, and dUTP incorporation for strand specificity.
Thanks a lot in advance!
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