Hi,
I was just wondering if anyone has ever seen a Eukaryote RNA Nano BioAnalyzer with an extremely large 28S peak. The 28S reached 70 fluorescent units and the 18S reached about 15, which resulted in an rRNA ratio for 28s/18s of 8.0. No that is that not the RIN value, that is the value for the rRNA ratio. I have run hundreds of BioAnalyzer chips myself and seen a ton more, but I have never seen ratios this high. Has anyone else ever seen this?
This could just be me being super skeptical, but I don't know if I want to use these samples to make libraries even though everything else about the traces look perfect. Any advice/comments would be greatly appreciated!
Thanks!
I was just wondering if anyone has ever seen a Eukaryote RNA Nano BioAnalyzer with an extremely large 28S peak. The 28S reached 70 fluorescent units and the 18S reached about 15, which resulted in an rRNA ratio for 28s/18s of 8.0. No that is that not the RIN value, that is the value for the rRNA ratio. I have run hundreds of BioAnalyzer chips myself and seen a ton more, but I have never seen ratios this high. Has anyone else ever seen this?
This could just be me being super skeptical, but I don't know if I want to use these samples to make libraries even though everything else about the traces look perfect. Any advice/comments would be greatly appreciated!
Thanks!
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