Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Genome references database with full taxonomy

    Dear experts,

    Is there a database with fully sequenced reference genomes with taxonomy? Better if in one/many fasta-files with taxonomy in headers like
    >Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia-Shigella;Escherichia coli str. K-12 substr. DH10B

    There is 16S/18S rRNA database like arb-silva.de for that, but I couldn't find full genome database.

    If such a database does not exists, what is the simplest way to mine this data from other databases (assuming good programming skills)?

    Thank you!

  • #2
    Here's the ftp link for the NCBI taxonomy database:

    ftp://ftp.ncbi.nih.gov/pub/taxonomy/

    I'm not quite sure what you want to use this for. Using FASTA files with full taxa in the headers seems incredibly inefficient.

    If you have NCBI GI numbers for sequences, you can find out the taxon for that sequence:

    ftp://ftp.ncbi.nih.gov/pub/taxonomy/gi_taxid.readme

    And then use the nodes.dmp file from the taxdump database to traverse the complete taxonomy:

    ftp://ftp.ncbi.nih.gov/pub/taxonomy/taxdump_readme.txt

    I guess you could also use the gi_taxid to do a reverse lookup, retrieving all the NCBI sequence GIs with a particular taxid.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    18 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    22 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    16 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    47 views
    0 likes
    Last Post seqadmin  
    Working...
    X