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Old 05-06-2015, 02:40 AM   #7
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Location: Germany

Join Date: Oct 2008
Posts: 414

Hi Brian,
I wouldn't say plant introns are as short as 16000. We have observed reliable introns as large as 50kbp in one species (confirmed by spliced alignments of multiple RNA-seq datasets) and 150kbp in another (maize).

Great to hear BBmap works well on RNA-seq reads and auto detects splice sites though!
colindaven is offline   Reply With Quote