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Old 10-08-2018, 08:07 PM   #14
js0402
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Location: Malaysia

Join Date: Sep 2018
Posts: 2
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Quote:
Originally Posted by falconpunch View Post
If you're still wondering after a month...

Generally no, you should see a tight band with any "normal" 16S amplification. This is because you're amplifying one specific DNA segment (typically the V4 area of the gene coding for 16S ribosomal RNA) and while this segment is variable in sequence (the info you want to know with the analysis), it shouldn't vary in length (what a band on a gel would show).

Hope that helps!
Thank you for the reply! If possible, can I know the primer and pcr condition that you've been using ?
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