We'd like to do a 454 transcriptome experiment on some primary, cultured cells and we were planning to just send total RNA to 454. They're asking for 50ug
On a good day we might get 10ug from a large flask - so how much total RNA is required for a transcriptome experiment? Having trouble getting a straight answer from anywhere and I'm pretty sure 50ug was never mentioned in our initial enquiries...
On a good day we might get 10ug from a large flask - so how much total RNA is required for a transcriptome experiment? Having trouble getting a straight answer from anywhere and I'm pretty sure 50ug was never mentioned in our initial enquiries...
Comment