Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • IndexOutOfBoundsException in BBTool's kmercountexact.sh

    I'm trying to use BBTool's kmercountexact.sh (version 37.50) to produce a sketch from a test fasta file:
    kmercountexact.sh in=test1.fasta sketch=test1.sketch mincount=3 -overwrite=true

    and I'm getting an IndexOutOfBoundsException. Does anyone have any idea of what I can do differently or how I may otherwise get help?

    Here is the full output:

    > kmercountexact.sh in=test1.fasta sketch=test1.sketch mincount=3 -overwrite=true
    Max memory cannot be determined. Attempting to use 3200 MB.
    If this fails, please add the -Xmx flag (e.g. -Xmx24g) to your command,
    or run this program qsubbed or from a qlogin session on Genepool, or set ulimit to an appropriate value.
    java -ea -Xmx3200m -Xms3200m -cp /Users/droe/bin/bbmap/current/ jgi.KmerCountExact in=test1.fasta sketch=test1.sketch mincount=3 -overwrite=true
    Executing jgi.KmerCountExact [in=test1.fasta, sketch=test1.sketch, mincount=3, -overwrite=true]

    Initial:
    Memory: max=3215m, free=3182m, used=33m

    Executing kmer.KmerTableSet [in=test1.fasta, sketch=test1.sketch, mincount=3, -overwrite=true]

    Initial:
    Ways=31, initialSize=128000, prefilter=f, prealloc=f
    Memory: max=3215m, free=3182m, used=33m

    Estimated kmer capacity: 121523321
    After table allocation:
    Memory: max=3215m, free=3111m, used=104m

    After loading:
    Memory: max=3215m, free=3060m, used=155m

    Input: 1 reads 1000 bases.

    For K=31
    Unique Kmers: 970
    Load Time: 0.059 seconds.
    Generating sketch.
    Exception in thread "main" java.lang.IndexOutOfBoundsException: Index: 0, Size: 0
    at java.util.ArrayList.rangeCheck(ArrayList.java:653)
    at java.util.ArrayList.get(ArrayList.java:429)
    at sketch.SketchTool.toSketch(SketchTool.java:327)
    at sketch.SketchTool.toSketch_MT(SketchTool.java:225)
    at sketch.SketchTool.toSketch(SketchTool.java:167)
    at jgi.KmerCountExact.makeSketch(KmerCountExact.java:360)
    at jgi.KmerCountExact.process(KmerCountExact.java:264)
    at jgi.KmerCountExact.main(KmerCountExact.java:58)
    Attached Files

  • #2
    I just tested and was able to replicate this... it's due to the "mincount=3" flag. That file has no kmers that occur at least 3 times, so it ends up trying to make a size-0 sketch, which isn't allowed. If you take away "mincount=3" it works fine. I'll clarify that error message.

    Comment


    • #3
      Makes sense. Thank you!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      27 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      31 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      27 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      52 views
      0 likes
      Last Post seqadmin  
      Working...
      X