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Old 06-04-2015, 07:19 AM   #2
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Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 206

never tried with total RNA below 10 pg but I can tell you that even T or B cells work (and they have VERY little mRNA!), you just have to increase the number of cycles a bit (21-22). In that case, you might have also to decrease the amount of primers and block all the oligos with biotin at the 5-end, to avoid getting just adaptor dimers.
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