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Old 11-27-2013, 07:58 AM   #22
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Location: Tampa, Florida

Join Date: Aug 2011
Posts: 7


my understanding is:
when you do PCR with two primers using the first strand cDNA as template, at first cycle it will use one of the primers (complimentary to the first strand cDNA) to generate a reverse complimentary strand that is actually is the second strand cDNA, and the following cycles will use the double strand DNA as template. How does the PCR only amplify the the first strand cDNA????

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