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Old 11-27-2013, 08:11 AM   #23
TonyBrooks
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Location: London

Join Date: Jun 2009
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Both strands are created in the PCR, but by digesting the second strand you ensure that the P5 adapter is always on the 5' end of the mRNA and P7 is always on the 3' end. This means all reads from the sequencer will be the same sequence as the mRNA that made the library (Illumina read 1 are read P5->P7)

http://www.rna-seqblog.com/wp-conten...reparation.jpg

Last edited by TonyBrooks; 11-27-2013 at 08:12 AM. Reason: including image
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