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Old 06-05-2014, 02:10 PM   #1
Location: Dallas

Join Date: May 2011
Posts: 10
Default which kit for RRBS?

Any advice on which kit to use for RRBS? I'm looking at Epignome Methyl-Seq; NuGen Ovation Ultra low Methyl-seq; Bioo NEXTflex bisulfite sequencing kit,
The Illumina web site is confusing - their RRBS workflow includes ligation of methylated adapters which are no longer available. I called to ask about the Epignome kit for RRBS and was advised not to do RRBS at all and use microarrays (or WGBS - which is cost prohibitive for a new lab start-up). Any advice for a newbie to RRBS would be much appreciated

Ultimately I want to use FFPE samples - impossible?
lyndaben is offline   Reply With Quote