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Old 03-13-2015, 06:59 AM   #5
Location: Dallas

Join Date: May 2011
Posts: 10

The new kit will be better hopefully. A note of caution to anyone using the old kit with the modified RRBS protocol: The first sequencing run we multiplexed with RNAseq samples and everything was great and the data looks good. This last time we multiplexed with PhiX (in 6 of 8 lanes) as recommended by NuGEN and we are having problems with the data (only 3-10M reads for the RRBS samples). On the same flow cell, two of the lanes were multiplexed with RNAseq and we got 30M reads for the RRBS samples, so we know it was not a sequencing problem or a library problem.
lyndaben is offline   Reply With Quote