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Old 08-22-2014, 01:00 PM   #2
nimitjain
Junior Member
 
Location: California

Join Date: Aug 2014
Posts: 1
Default Cufflinks Mask Option?

Hi Xianjun

Were you able to figure out how the cufflinks mask option works?

I am myself trying to figure this out. In particular, I am confused whether the masked transcripts are excluded from the FPKM denominator. I tried running cufflinks with and without the mask option on the same dataset and the FPKM values for my genes of interest didn't change much, leading me to believe that the masked transcripts are still factored into the FPKM denominator but perhaps not included in estimation of the fragment size distribution (based on how I created the mask file, I should have seen a difference in the FPKM values if the masked transcripts were being excluded from the FPKM denominator).

Any insights?

Nimit
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