We are working on sequencing human exome using agilent for enrichment and GAIIx for sequencing.
We got a high heterogeneity of read profiles from a DNA sample to an other.
For example : for a given mutation, we got for 1 DNA sample a deph of 50X, for an other it will be 2X and so on.
Is it a problem due to Agilent or Illumina?
Did anybody read a paper about this issue?
We got a high heterogeneity of read profiles from a DNA sample to an other.
For example : for a given mutation, we got for 1 DNA sample a deph of 50X, for an other it will be 2X and so on.
Is it a problem due to Agilent or Illumina?
Did anybody read a paper about this issue?