Hallo all,
What I allways wanted to know is why you need to add the nucleotides one by one when using helicos. I know they use the same dye for each nucleotide, but why is this? Why not use 4 different dyes like when using solexa you can add all 4 labeled nucleotides at the same time.
Is this because in helicos you did not have an amplification step and less DNA (no clustering) and thus you need to be more "carefull" with measuring the fluorescence?
Thanks in advance
What I allways wanted to know is why you need to add the nucleotides one by one when using helicos. I know they use the same dye for each nucleotide, but why is this? Why not use 4 different dyes like when using solexa you can add all 4 labeled nucleotides at the same time.
Is this because in helicos you did not have an amplification step and less DNA (no clustering) and thus you need to be more "carefull" with measuring the fluorescence?
Thanks in advance
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