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Old 08-12-2017, 10:06 PM   #6
smurugesan
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Location: Doha, Qatar

Join Date: Aug 2017
Posts: 6
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Thank you Jessica and Brian for your valuable suggestions. In Illumina guide, they suggested to sequence an amplicon of 550bp or smaller. So I thought it would be ideal, and actually We are planning to sequence V1-V3 regions. In addition, I would like to ask your expertism. I am new to this technology, By using barcodes only in forward primers and common reverse primer for all the sample, Is it possible to distinguish the sequences read through reverse primer to do overlapping? I am not clear with it. If anybody explain this part, it would be great. Thank you.
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