View Single Post
Old 04-25-2018, 09:48 AM   #5
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,300
Default

Quote:
Originally Posted by nucacidhunter View Post
TruSeq kits based on ligating duel indexed adapters (either combinatorial or unique) do not use indexed primers.
Although, a little weirdly, the Epicentre "scriptseq" kit is sold by Illumina and that uses step-out PCR to add the indexes, etc.

Well, not indexes but "index"-- I don't think ScriptSeq uses dual indexing natively. But because the final products are TruSeq-identical it is trivial to design UDI's for them.

Also, it looks like Swift Bioscience has a UDI kit as well?

--
Phillip
pmiguel is offline   Reply With Quote