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Old 10-24-2017, 02:48 PM   #1
hoytpr
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Location: Stillwater

Join Date: Dec 2009
Posts: 47
Default Client sends wrong index. Run is done.

We had a client send us a set of 43 multiplexed samples. Although the NextSeq500 run went well, only 54% of the reads passed filter (PF). We normally get in the 93%+ range, so we dug but couldn't find any explanation except that there must have been a bad index (typo) in the sample sheet. Not our error but hey, we want to help.

Some of the samples had essentially zero reads after demultiplexing. We also found discarded reads where over a million had the same index.

Question: Can we easily determine which samples need to be re-de-multiplexed? This must have happened before.

Thanks,
-pete

Last edited by hoytpr; 10-24-2017 at 02:49 PM. Reason: added instrument
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