Dear All, i am working on a project that will do De novo RNA-Seq Assembly using Trinity. Totally have 16 samples from 2 projects.
1. 8 sample, PE150, 30x, Hiseq 2500
2. another 8 sample, PE300, 100x, Miseq
I am wondering that if the Trinity can take the reads with variable length reads? and is there any technical and biological differences impact on the result if the data from a different condition, platform, and reads lengths?
Can you please give me some pointers? Appreciate it.
1. 8 sample, PE150, 30x, Hiseq 2500
2. another 8 sample, PE300, 100x, Miseq
I am wondering that if the Trinity can take the reads with variable length reads? and is there any technical and biological differences impact on the result if the data from a different condition, platform, and reads lengths?
Can you please give me some pointers? Appreciate it.
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