Dear all,
I would like to put double index on Illumina library to avoid PCR jumping and to pool samples together for gene capture. I remember there was a paper about putting index on the insert instead of on the adapters a few years back. I think it is better, because sequencing double index in not very popular, so it is hard to find enough samples for sharing a flow cell. I searched all my files and google sites and pulled all my hair and still can't find the paper I read before. Could anyone give me a clue of the paper I missed, and what do you think is the best strategy to solve the problem. Thanks in advance.
Chenhong
I would like to put double index on Illumina library to avoid PCR jumping and to pool samples together for gene capture. I remember there was a paper about putting index on the insert instead of on the adapters a few years back. I think it is better, because sequencing double index in not very popular, so it is hard to find enough samples for sharing a flow cell. I searched all my files and google sites and pulled all my hair and still can't find the paper I read before. Could anyone give me a clue of the paper I missed, and what do you think is the best strategy to solve the problem. Thanks in advance.
Chenhong
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