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Old 04-23-2012, 03:02 PM   #4
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Location: SF Bay Area, CA, USA

Join Date: Oct 2007
Posts: 1,358

Can't resist responding to this...ease of preparation goes hands down, no contest, to the MiSeq.

Won't expand too much...but suffice to say that you can be sequencing your library on the MiSeq 15 minutes after it's quantitated. With the PGM you still have at least several hours of hands on manual labor (instrument prep, emulsion setup, enrichment, bead quantitation, chip prep/loading, etc), that involve two other pieces of instrumentation.

Not to mention you can set a MiSeq on a bench, plug it in and it works. Doesn't need uber-grade water, a constant supply of argon, or a giant server.
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