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Old 04-24-2012, 03:41 PM   #9
snetmcom
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Location: USA

Join Date: Oct 2008
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Quote:
Originally Posted by ECO View Post
Can't resist responding to this...ease of preparation goes hands down, no contest, to the MiSeq.

Won't expand too much...but suffice to say that you can be sequencing your library on the MiSeq 15 minutes after it's quantitated. With the PGM you still have at least several hours of hands on manual labor (instrument prep, emulsion setup, enrichment, bead quantitation, chip prep/loading, etc), that involve two other pieces of instrumentation.

Not to mention you can set a MiSeq on a bench, plug it in and it works. Doesn't need uber-grade water, a constant supply of argon, or a giant server.
It's not quite several hours. More like an hour or so.

Can anyone comment on miseq analysis times. Does it do alignments? I haven't used one in many months and at the time the software was still dealing with windows issues.
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